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KMID : 0363219720100030153
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Korean Journal of Dermatology
1972 Volume.10 No. 3 p.153 ~ p.164
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The Comparative Studies on the Antigenicity of Antidog Spleen, Lymphnode and Thymus Sera in Rabbit.
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Abstract
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The present study was undertaken to evaluate and compare the in vitro leucoagglutination titers and in vivo lymphopenic effect of three different antilymphocytic sera.
Rabbits were immunized against adult, dog and pup lymphoid tissue with multiple intramuscular injections of pooled spleen, mesenteric lymphnode and thymus cells. The number of lymphoid cells per each immunization was 1.8 x 10^(8) of spleen cells, 1.68x10^(8) of mesenteric lymphnode cells and 4.4 x 10^(7) of thymus cells and immunization was done once per 10 days for 2 months. After completion of immunization and one booster injection, the rabbits were bled and total of 11 antidog spleen sera(RADSS), 4 antidog lymphnode sera (RADLS) and 3 antidog thymus sera (RADTS) were prepared and stored at-20℃ until used.
With in vitro agglutination technique, each serum was serially diluted with buffered saline and tested with freshly prepared dog spleen, lymphnode, thymus cells and peripheral blood buffy coat leucocytes. With in vivo test, the antilymphocytic sera were intramuscularly injected in a dose of 1 ml/kg into adult dogs and after 12 hours peripheral blood pictures were evaluated and compared with those before antisera administration. The results were as follows.
1. The range of in vitro leucoagglutination titers of RADSS for spleen cells, lymphnode cells, thymus cells and peripheral leucocytes was 1 : 4 to 1 : 128, 1 : 4 to 1 : 128, 1 : 4 to 1 : 64 and 1 : 4 to 1 : 128, respectively.
2. The range of in vitro leucoagglutination titers of RADLS for spleen cells, lymphnode cells, thymus cells and peripheral leucocytes was 1 : 32 to 1 : 64, 1 : 32 to 1 : 256, 1 : 32 to 1 : 128 and 1 : 16 to 1 : 256, respectively.
3. The range of in vitro leucoagglutination titers of RADTS for spleen cells, lymphnode cells, thymus cells and peripheral leucocytes was 1 : 16 to 1 : 128, 1 : 16 to 1 : 64, 1 : 8 to 1 : 64 and 1 : 8 to 1 : 64, respectively.
4. After a single intramuscular injection of the antisera, the total R.B.C. counts were not significantly changed in RADSS, RADLS and RADTS. However, the percentage of neutrophils increased by a mean value of 35.3%, 34.8% and 21.7% in RADSS, RADLS and RADTS, respectively and the percentage of lymphocytes decreased by a mean value of 47.8 %, 51.8% and 30.6%, in RADSS, RADLS and RADTS, respectively.
The eosinophils showed a tendency of decrease in RADSS, RADLS and RADTS, respectively. The monocytes and basophils were not significantly changed after injection of the antisera.
The author found that there was no significant difference between the in vitro leucoagglutination titers of three kind of antisera and that with in vitro leucoagglutination test, the peripheral blood leucocytes may serve as an useful antigen because of simplicity of preparation. Also, the author found that there was no direct correlation between the in vitro leucoagglutination titers and in vivo lymphopenic effect, but generally the antisera with strong lymphopenic effect had higher in vitro titers than those with weak lymphopenic effect. In this experiment, the lymphopenic effect of RADTS was slightly lower than those of RADSS and RADLS and this difference may be due to smaller number of the cells used in immunization for the preparation of the antisera. The author concluded that spleen was as useful as lymphnode or thymus in its antigenicity and in producing effective antilymphocytic serum.
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